Re: pinhole-thickness

Posted by mmodel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/pinhole-thickness-tp593574p593575.html

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Hi Valeria

I don't think there's a simple formula, but in the 2nd edition of
Confocal Handbook there is a chapter by Wilson that talks about the
effect of pinhole on resolution.

Mike

Michael Model, Ph.D.
Confocal Microscopy Core
Dpt. Biological Sciences
Kent State University
Kent, OH 44242
tel. [phone]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Valeria Berno
Sent: Tuesday, July 08, 2008 7:47 AM
To: [hidden email]
Subject: pinhole-thickness

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Dear all,

I know this is a very basic question but I am having hard time to find
the
answer.

How can I calculate the thickness of the optical section I am aquiring
with different pinhole size?

So waht is its relation with objective resolution?

Thanks in advance

Valeria