Re: pinhole-thickness

Posted by Eric Scarfone on
URL: http://confocal-microscopy-list.275.s1.nabble.com/pinhole-thickness-tp593574p593578.html

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Valeria
I would say that the size of your optical section is entirely
dependant on the NA of your objective. What happens when you open your
pinhole is that you let photons coming from "outside" contribute to
image formation. Of course, you also get back difracted light coming
from your focal plane that you otherwise you would have rejected.
The end effect is more a blurring than a "thickening".
the simplest solution is to get a infra red pulsed laser! No need of
pinhole with multiphoton!
Cheers
Eric

Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
Department of Clinical Neuroscience
Karolinska Institutet

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----- Original Message -----
From: Valeria Berno <[hidden email]>
Date: Tuesday, July 8, 2008 1:47 pm
Subject: pinhole-thickness
To: [hidden email]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear all,
>
> I know this is a very basic question but I am having hard time to
> find the
> answer.
>
> How can I calculate the thickness of the optical section I am

aquiring
> with different pinhole size?
>
> So waht is its relation with objective resolution?
>
> Thanks in advance
>
> Valeria
>