question about plate readers

Posted by Aryeh Weiss on
URL: http://confocal-microscopy-list.275.s1.nabble.com/reducing-size-of-illumination-in-epi-fluorescence-tp593586p593592.html

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Do plate readers (ie, machines that are supposed to detect fluorescence in 96
well plates) detect fluorescence in cells that are adherent to the bottom of the
wells, or is the fluorescence detected mainly  from the solution in the wells?

I would have thought that the detect total fluorescence in a volume, and in
principle it would not matter if the fluorescence is contained in adherent
cells, or in the solution. However, recently  we have the experience of seeing
cells clearly with bright fluorescence in a microscope, and then getting no
reading from the plate reader.

I have very little experience with plate readers, so the collective wisdom of
the group will be greatly appreciated.

--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]