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> I am a PhD student at Auburn University just getting started into my
> thesis work. I am
> looking for a reliable method for quantifying fluorescence.
>
> My project deals with marine dinoflagellates (zooxanthellae) that
> reside intracellularly in
> hosts (usually cnidarians). I am working with cultures or isolates
> of only the dinoflagellates
> for my confocal work. I have an antibody that was created against
> the surface secretions
> of mucilage (secreted as part of a daily cycle by the alga) from one
> strain of zooxanthellae.
> I am attempting to use this antibody to label various strains to
> identify differences in
> mucilage between them. Thus far, I have seen that the strain the
> antibody was created
> against labels around the cell fairly brightly. Most samples either
> show this or a complete
> lack of labeling. However, a few samples show a faint fluorescence
> lifted off the cell
> surface. I would like to be able to quantify this fluorescence in
> comparison to either the
> control strain that the antibody was created against or against a
> known fluorescence.
>
> Any help on ideas for this, or places to look for ideas would be
> greatly appreciated.
>
> Thanks!
>
> Maria Mazzillo
>
Dep. Moleculaire Biotechnologie