DiD / DiI / DiO versions 'solid' and 'oil'

Posted by Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/sorry-tp593651p593652.html

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Hello there,

the DiI / DiO related membrane stain DiD from
Molecular Probes comes in two flavors (as do its relatives):

'DiD' oil = DiIC18(5) oil (cat# D307) or as 'DiD'
solid = DiIC18(5) solid (cat# D7757).

Can anyone comment on what the
advantages/disadvantages of the two flavors are?

The idea for the experiment is to use DiD to
stain the membranes of endothelial cells of blood
vessels (from the interior of the vessel), to be
able to distinguish the endothelial cells better
from neighboring smooth muscle cells (SMCs)
Povided that the endothelium is intact, the dye
should not be able to reach the SMCs - or so ist
the hope. DiD should be sufficiently in the red
to allow the simultaneous usage of the green
channel e.g. for Fluo-4 Ca measurements.

Regards

Steffen

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Steffen Dietzel, PD Dr. rer. nat
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