Embedding Arabidopsis Thaliana for confocal

Posted by annaoru on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Embedding-Arabidopsis-Thaliana-for-confocal-tp5954747.html

Hi!
I´m a student doing my exam-project where we are using Arabidopsis Thaliana to express a HIV protein (p24) in order to creat an edible vaccine.
Previously I have fixated and embedded (paraffin wax) the tissues to vizualise it in Light microscope (via IHC). Now I need to do the same procedure but try to analyze the tissue using confocal microscope. For that I need to know if I can use the same fixation solution for confocal as I did for Light microscope which was: egual volume of 0.2M Sodiumphosphate buffer pH 7.2 and Paraformaldehyde (8%) solution?
And also what kind of embedding should I use for the confocal?
Since I have a lot of material left from my previous work I´m hoping to be able to use it as it is for the confocal.
Is this possible?

Thank you for replying!

/Anna