RE: Embedding Arabidopsis Thaliana for confocal
Posted by
annaoru on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Embedding-Arabidopsis-Thaliana-for-confocal-tp5954747p5957664.html
Okej George!
How interesting!
How is it working and if I would use that technique can a re-use the tissue I already have fixated and embedded or do I need fresh tissue?
Thank you for helping!
Regards
/Anna
Date: Mon, 24 Jan 2011 17:16:57 -0800
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Hi Anna,
If you are happy with the morphology of your IHC sections, my
fluorescent tyramide suggestion will cost you some money to purchase the
kit, but enable you to avoid other changes.
best wishes,
George
On 1/24/2011 4:16 PM, annaoru wrote:
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>
> Hi!
> No I haven´t tried yet but I think I might try just to see the results.
> I haven´t succeeded to find any information regarding this and the
> possibilities around using the same embedding and fixation technique as I
> did prior to Light microscopy but I hope that it will work or that I at
> least can use the same fixation so that the embedded tissue can be melt and
> re-embedded in resin instead. What do you think about that?
>
> Thank you for helping!
>
> Regards!
> /Anna
>
--
George McNamara, PhD
Analytical Imaging Core Facility
University of Miami
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