Photoactivation of PA-tagRFP by two-photon excitation

Posted by hanneke okkenhaug (BI) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Photoactivation-of-PA-tagRFP-by-two-photon-excitation-tp5962484.html

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Hi.
I was wondering if anyone has any experience using two photon excitation to photoactivate PA-tagRFP (or similar red fluorescent protein)?
I am getting it to activate fine in confocal mode with the 405nm laser, but am struggling to get the right settings (wavelength, power, dwell time) to activate by 2P.

Thanks
Hanneke

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Hanneke Okkenhaug, PhD
Laboratory of Molecular Signalling
Babraham Institute
Cambridge CB22 3AT
United Kingdom
Phone: [phone]
Fax:        [phone]
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