Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Who-has-purchased-a-fluorescence-nanoscope-tp5972858.html
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Dear Confocal listserv,
Who has purchased a fluorescence nanoscope? What can you tell me about
your experiences - either here or privately?
I see 11 OMX labs listed at
http://www.api.com/omx-labs.asp (may be out
of date or some customers may be shy).
I am aware of STED systems in the USA at Yale Univ, NIH and I was told
San Diego (have not found where), and possibly UC Denver. Paul French
apparently did his own upgrade of a Leica SP2
(
http://www.focusonmicroscopy.org/2008/PDF/070_Auksorius.pdf and a Leica
Scientific Forum video ... see also related work at
ftp://ftp.espci.fr/incoming/Gilles/INSERM-STED-x6.pdf ).
I am also aware of one 4pi microscope in the USA.
So, who has N-SIM, N-STORM, SR-SIM, PALm, SR-200, and what can you tell
me about your experiences with your (purchased) nanoscope(s)?
Sincerely,
George
p.s. Please no need to clutter up the listserv with other people's
nanoscope references - I know how to use PubMed. For that matter, I've
replicated the results of the following two papers on my confocal's:
Subdiffraction fluorescence imaging of biomolecular structure and
distributions with quantum dots. </pubmed/20600360>
Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D,
Kaltschmidt B, Kaltschmidt C, Heilemann M.
Biochim Biophys Acta. 2010 Oct;1803(10):1224-9. Epub 2010 Jun
23.PMID: 20600360
Quantum dot triexciton imaging with three-dimensional subdiffraction
resolution. </pubmed/19453186>
Hennig S, van de Linde S, Heilemann M, Sauer M.
Nano Lett. 2009 Jun;9(6):2466-70.PMID: 19453186
I have used <1 Airy unit pinhole to improve triexciton resolution
further. I have not tried doing 3D deconvolution on the data.
--
George McNamara, PhD
Analytical Imaging Core Facility
University of Miami