Re: Who has purchased a fluorescence nanoscope?

Posted by lechristophe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Who-has-purchased-a-fluorescence-nanoscope-tp5972858p5972966.html

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The Bordeaux Imaging Center in Bordeaux, France has a STED and a TIRF/PALM
http://www.bic.u-bordeaux2.fr/

PALM/STORM setups are becoming more common (I'm aware of 2 setups already
running in Marseille), because (at least this is what the people who did it
told me) it is quite easy to add to an existing TIRF setup (provided you
find software for the detection and localisation of individual fluorophores,
but there is now  an available ImageJ plugin for that).


--
Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
[hidden email]




On Sat, Jan 29, 2011 at 16:52, G. Esteban Fernandez <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> UCLA has a STED
>
> http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791
> <http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791>
> http://www.cnsi.ucla.edu/staticpages/core-facilities#alms
>
>
> <http://www.cnsi.ucla.edu/staticpages/core-facilities#alms>
>
> On Sat, Jan 29, 2011 at 7:32 AM, George McNamara
> <[hidden email]>wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear Confocal listserv,
> >
> > Who has purchased a fluorescence nanoscope? What can you tell me about
> your
> > experiences - either here or privately?
> >
> > I see 11 OMX labs listed at http://www.api.com/omx-labs.asp (may be out
> of
> > date or some customers may be shy).
> >
> > I am aware of STED systems in the USA at Yale Univ, NIH and I was told
> San
> > Diego (have not found where), and possibly UC Denver. Paul French
> apparently
> > did his own upgrade of a Leica SP2 (
> > http://www.focusonmicroscopy.org/2008/PDF/070_Auksorius.pdf and a Leica
> > Scientific Forum video ... see also related work at
> > ftp://ftp.espci.fr/incoming/Gilles/INSERM-STED-x6.pdf ).
> >
> > I am also aware of one 4pi microscope in the USA.
> >
> > So, who has N-SIM, N-STORM, SR-SIM, PALm, SR-200, and what can you tell
> me
> > about your experiences with your (purchased) nanoscope(s)?
> >
> > Sincerely,
> >
> > George
> > p.s. Please no need to clutter up the listserv with other people's
> > nanoscope references - I know how to use PubMed. For that matter, I've
> > replicated the results of the following two papers on my confocal's:
> >
> >   Subdiffraction fluorescence imaging of biomolecular structure and
> >   distributions with quantum dots. </pubmed/20600360>
> >
> >   Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D,
> >   Kaltschmidt B, Kaltschmidt C, Heilemann M.
> >
> >   Biochim Biophys Acta. 2010 Oct;1803(10):1224-9. Epub 2010 Jun
> >   23.PMID: 20600360
> >
> >
> >   Quantum dot triexciton imaging with three-dimensional subdiffraction
> >   resolution. </pubmed/19453186>
> >
> >   Hennig S, van de Linde S, Heilemann M, Sauer M.
> >
> >   Nano Lett. 2009 Jun;9(6):2466-70.PMID: 19453186
> >
> > I have used <1 Airy unit pinhole to improve triexciton resolution
> further.
> > I have not tried doing 3D deconvolution on the data.
> >
> >
> > --
> >
> >
> > George McNamara, PhD
> > Analytical Imaging Core Facility
> > University of Miami
> >
>