Posted by
kspencer007 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-Objective-lens-chromatic-aberration-tp5929621p6014056.html
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Thanks Ippei;
We are imaging 2-3 days, and the reservoir is drying out. Short term, it does seem OK. I'm just unsure about why it is drying out, if we are just imaging much longer than normal. If we put in wet Kimwipes, everything seems fine. Somehow, we need to add water during our imaging.
Kathy
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Ippei Kotera
Sent: Thursday, February 10, 2011 3:56 PM
To:
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Subject: Re: tokai hit chamber
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Hi Kathy,
Tokai Hit System I had been using was equipped with a doughnut-shaped
water chamber and heated lid along with CO2 and heat supplier. I have
done live imaging up to 5 hours with no humidity problem. What kind of
humidity issues do you have? The water chamber was simple enough that
nothing could go wrong, so it seemed.
Ippei
--
Ippei Kotera, Ph.D.
Tanz CRND
Tanz Neuroscience Building, Room 221
University of Toronto
6 Queen's Park Crescent West
Toronto, Ontario M5S 3H2
Phone: (416)978-2503
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