Posted by
Romin, Yevgeniy/Sloan Kettering Institute on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-Objective-lens-chromatic-aberration-tp5929621p6014260.html
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Hi Kathy
We have several Tokai Hit icubators in our facility, they all dry out within 24 hours, the company seems to be aware of it and they suggest to put in more water every 12 hours, so that is what we do. Wet Kimwipes also help. Or wet cotton balls.
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From: Confocal Microscopy List [
[hidden email]] On Behalf Of Kathryn Spencer [
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Sent: Thursday, February 10, 2011 7:17 PM
To:
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Subject: Re: tokai hit chamber
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Thanks Ippei;
We are imaging 2-3 days, and the reservoir is drying out. Short term, it does seem OK. I'm just unsure about why it is drying out, if we are just imaging much longer than normal. If we put in wet Kimwipes, everything seems fine. Somehow, we need to add water during our imaging.
Kathy
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From: Confocal Microscopy List [mailto:
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Sent: Thursday, February 10, 2011 3:56 PM
To:
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Subject: Re: tokai hit chamber
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Hi Kathy,
Tokai Hit System I had been using was equipped with a doughnut-shaped
water chamber and heated lid along with CO2 and heat supplier. I have
done live imaging up to 5 hours with no humidity problem. What kind of
humidity issues do you have? The water chamber was simple enough that
nothing could go wrong, so it seemed.
Ippei
--
Ippei Kotera, Ph.D.
Tanz CRND
Tanz Neuroscience Building, Room 221
University of Toronto
6 Queen's Park Crescent West
Toronto, Ontario M5S 3H2
Phone: (416)978-2503
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