http://confocal-microscopy-list.275.s1.nabble.com/Re-Objective-lens-chromatic-aberration-tp5929621p6015889.html
FYI- Our Pathology Devices stage incubator does not have this problem and works for days with little media loss. Dave
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> Great advice. Thank you.
> Kathy
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> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Yevgeniy Romin
> Sent: Thursday, February 10, 2011 5:54 PM
> To:
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> Subject: Re: tokai hit chamber
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> Hi Kathy
>
> We have several Tokai Hit icubators in our facility, they all dry out within 24 hours, the company seems to be aware of it and they suggest to put in more water every 12 hours, so that is what we do. Wet Kimwipes also help. Or wet cotton balls.
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> From: Confocal Microscopy List [
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> Sent: Thursday, February 10, 2011 7:17 PM
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> Subject: Re: tokai hit chamber
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> Thanks Ippei;
> We are imaging 2-3 days, and the reservoir is drying out. Short term, it does seem OK. I'm just unsure about why it is drying out, if we are just imaging much longer than normal. If we put in wet Kimwipes, everything seems fine. Somehow, we need to add water during our imaging.
> Kathy
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> -----Original Message-----
> From: Confocal Microscopy List [mailto:
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> Sent: Thursday, February 10, 2011 3:56 PM
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> Subject: Re: tokai hit chamber
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> Hi Kathy,
>
> Tokai Hit System I had been using was equipped with a doughnut-shaped
> water chamber and heated lid along with CO2 and heat supplier. I have
> done live imaging up to 5 hours with no humidity problem. What kind of
> humidity issues do you have? The water chamber was simple enough that
> nothing could go wrong, so it seemed.
>
> Ippei
>
> --
> Ippei Kotera, Ph.D.
>
> Tanz CRND
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