Posted by
Andreas Bruckbauer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolution-of-3D-SIM-data-tp6251420p6254248.html
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When i generate a PSF for deconvolution using suitable beads, then image the same beads again and deconvolve the image, i would expect to get really tiny dots. See e.g.
http://www.svi.nl/BeadsDeconvolutionExample
I would expect something similar for the OMX when the reconstruction is perfect and includes proper deconvolution. However we get larger features, still within the expected OMX resolution 120 nm in width and 300 nm in z, but no dots. I think there is still some improvement possible either in the setup of the instrument or the software.
best wishes
Andreas
-----Original Message-----
From: Hans <
[hidden email]>
To:
[hidden email]
Sent: Fri, 8 Apr 2011 10:46
Subject: Re: Deconvolution of 3D SIM data
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(*commercial response*)
Dear All,
As the previous responses by David and Dan already state very well, caution should be taken in straightforward applying deconvolution on top of reconstructed images from SIM systems since these might be the result of various non-linear processing steps.
Still, since it seems that there is room for improvement in at least some SIM reconstructions, and the current discussion also points in that direction, here at SVI we wonder about the need for a SIM deconvolution module. This would work directly on raw SIM data.
We would very much appreciate to get some off-listfeedback from (potential) SIM users for such a module.
Cheers, Hans
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dr. Hans T.M. van der Voort (
[hidden email])
Scientific Volume Imaging b.v., URL:
http://www.svi.nl/