Posted by
Gert van Cappellen-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolution-of-3D-SIM-data-tp6251420p6255144.html
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The problem with reconstruction of the SIM images is I think as with
most other imaging techniques the signal to noise ratio of the raw
images. An optimal deconvolution should there start on the raw images
and not on the reconstructed images. As stated before all SIM algorithms
do already contain some deconvolution, but I think there is room for
improvement. With a better knowledge of the deformations of the grid in
the images and the effect in biological specimen the reconstruction
based on deconvolution might improve. This needs a lot of testing with
different biological samples or other tricks.
With kind regards,
Gert van Cappellen
Optical Imaging Centre
Erasmus MC (Erasmus Medical Centre)
Rotterdam
Netherlands
Op 7-4-2011 22:16, Amro Hamdoun schreef:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hi Everyone,
>
> We are starting to process through OMX data sets and we are writing for any
> thoughts on the use of deconvolution in these images. We have heard mixed
> things about whether or not it is valid... Has anyone here deconvolved 3D SIM
> data? Were actual or theoretical PSFs used?
>
> Much thanks,
> A