Re: Deconvolution of 3D SIM data

Posted by Guy Cox-2 on Apr 13, 2011; 10:28pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolution-of-3D-SIM-data-tp6251420p6270857.html

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Abbe considered rays diffracted by two points on the sample.  The points
will be resolved if their diffracted rays can enter the objective.  This
can only apply to  a specimen illuminated from an external source.  In
fluorescence each point emits light and this is totally incoherent with
respect to light from another point.   There is no diffraction at the
sample so Abbe's calculation cannot be applied.  Rayleigh's criterion,
based on how the optics turn the image of a point into a disk (the Airy
disk) does apply.

 

 
Guy

 

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From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Martin Wessendorf
Sent: Thursday, 14 April 2011 12:10 AM
To: [hidden email]
Subject: Re: Deconvolution of 3D SIM data

 

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On 4/13/2011 7:33 AM, Guy Cox wrote:

> Abbe's limit,
> on the other hand, is non-negotiable, but it doesn't apply in
> fluorescence.

Guy--can you elaborate a bit on your statement that, "it doesn't apply
in fluorescence"?  Are you talking about STED, or talking in more
general terms?

Thanks--

Martin
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