Re: Deconvolution of 3D SIM data

Posted by Martin Wessendorf-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolution-of-3D-SIM-data-tp6251420p6271449.html

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On 4/13/2011 5:28 PM, Guy Cox wrote:

> Abbe considered rays diffracted by two points on the sample.  The points
> will be resolved if their diffracted rays can enter the objective.  This
> can only apply to  a specimen illuminated from an external source.  In
> fluorescence each point emits light and this is totally incoherent with
> respect to light from another point.   There is no diffraction at the
> sample so Abbe's calculation cannot be applied.  Rayleigh's criterion,
> based on how the optics turn the image of a point into a disk (the Airy
> disk) does apply.

This is (for me!) a very intuitive explanation, but it suggests that
with fluorescence, arbitrarily small resolution can be attained given
sufficiently high s/n.  That sounds something like what you said in your
earlier posting, except for the phrase "arbitrarily small".

Is that correct?  If not, what is the absolute limit of resolution in
fluorescence?

Martin

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