Re: Deconvolution of 3D SIM data

Posted by Martin Wessendorf-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolution-of-3D-SIM-data-tp6251420p6274318.html

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On 4/14/2011 3:31 PM, Mark Cannell wrote:

> I'm afraid the idea is MUCH older than 2008. I recall reading a paper
> from the (?) 1960's that discussed the idea that the diffraction limit
> was not a real limit at all. I believe they were using bacteria as an
> example of where knowledge of the object imparts more information to
> overcome the 'limit'

I think no one would deny that if you know what you're looking at, you
can break the "resolution barrier"--STORM and PALM being prime examples.
  However, I'd still be interested in hearing whether--without having
any additional information about the sample--there is any theoretical
limit to resolution in fluorescence microscopy, other than that imposed
by signal-to-noise ratio.

Martin
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