Re: Deconvolution of 3D SIM data

Posted by Ian Dobbie on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolution-of-3D-SIM-data-tp6251420p6281660.html

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Nuno Moreno <[hidden email]> writes:

> Therefore, I would be very, very skeptical either on any deconvolution
> and quantification using SIM.

Actually if your data has high S/N then quantitation should be
possible. For 2D super-resolution with SIM you have an over specified
linear problem, 9 images to give 4x the data (double resolution in
x&y). With 3D-SIM, as produced by OMX scopes, the problem is similar, 15
images producing 8x as much information (double res in x,y & z). Go look
at the original Gustafson papers for the details.

The issue is getting high enough S/N, which in practical terms means low
enough bleaching while taking those 100+ images.

Ian
--
Ian Dobbie
Micron Imaging Facility Manager,
Biochemistry,
University of Oxford,
South Parks Road,
Oxford
OX1 3QU
Tel: [phone]
Email: [hidden email]