Live cell imaging with multiphoton confocal

Posted by Knecht, David on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-with-multiphoton-confocal-tp6297623.html

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I have limited experience with multi-photon confocal microscopes.  I tried imaging live cells with one a while back and was unimpressed with the results as compared to a standard laser scanning instrument.  If you had a new single photon instrument and a new multi-photon instrument sitting side by side in your facility (and I presume some of you do), which would you choose for imaging  fixed cells or live cells in culture (not trying to go deep into tissue)?  Thanks- Dave

Dr. David Knecht    
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)