Re: Live cell imaging with multiphoton confocal
Posted by
Vladimir Ghukasyan-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-with-multiphoton-confocal-tp6297623p6297637.html
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Hi David,
For this kind of application MP has no advantage other than no
out-of-focus photobleaching. CLSM is a better solution.
With regards,
Vladimir Ghukasyan
Confocal and Multiphoton Imaging Facility
Neuroscience Center
University of North Carolina
115 Mason Farm Rd.,
Chapel Hill 27599-7250
Tel.: (919) 966 5807
On Fri, Apr 22, 2011 at 1:29 PM, David Knecht charter
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> I have limited experience with multi-photon confocal microscopes. I tried imaging live cells with one a while back and was unimpressed with the results as compared to a standard laser scanning instrument. If you had a new single photon instrument and a new multi-photon instrument sitting side by side in your facility (and I presume some of you do), which would you choose for imaging fixed cells or live cells in culture (not trying to go deep into tissue)? Thanks- Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
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