Posted by
Lemasters, John J. on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-with-multiphoton-confocal-tp6297623p6299607.html
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Multiphoton microscopy is helpful to excite UV-absorbing fluorophores like NAD(P)H that cannot otherwise be excited without specialized lasers and optics.
--
John J. Lemasters, MD, PhD
Professor and GlaxoSmithKline Distinguished Endowed Chair
Director, Center for Cell Death, Injury and Regeneration
Departments of Pharmaceutical & Biomedical Sciences and Biochemistry & Molecular Biology
Medical University of South Carolina
QF213 Quadrangle Building
280 Calhoun Street, MSC 140
Charleston, SC 29425
Office: 843-792-2153
Lab: 843-792-3530
Fax: 843-792-8436
Email:
[hidden email]
http://academicdepartments.musc.edu/ccdir-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Kate Luby-Phelps
Sent: Saturday, April 23, 2011 11:22 AM
To:
[hidden email]
Subject: Re: Live cell imaging with multiphoton confocal
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Multiphoton has no clear advantage for cells in culture. For cell culture samples, we use two photon only to excite DAPI or other UV and near-UV fluors since we had to make a choice between the Ti:S and a 405 laser on our scope.
Kate Luby-Phelps