Re: Live cell imaging with multiphoton confocal

Posted by Knecht, David on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-with-multiphoton-confocal-tp6297623p6300168.html

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Is there any disadvantage to multiphoton for cultured cells (besides cost and complexity)? Cell viability?  Phototoxicity?  Dave

On Apr 23, 2011, at 11:21 AM, Kate Luby-Phelps wrote:

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> Multiphoton has no clear advantage for cells in culture. For cell culture samples,
> we use two photon  only to excite DAPI or other UV and near-UV fluors since we
> had to make a choice between the Ti:S and a 405 laser on our scope.
>
> Kate Luby-Phelps

Dr. David Knecht    
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)