Re: post aquization binning clarification

Posted by Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-post-aquization-binning-clarification-tp6305795p6306430.html

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This is a bit tricky - summing could easily take pixels over the limit
and white out bits of your image.   If you bin while you are acquiring
you can adjust the gain to compensate, but for post-processing the
average approach seems much better.  If you then want to boost
brightness you can always multiply each pixel by a fixed amount (and if
you multiply by the binning size you'll exactly cancel out the
averaging).  

 

 
Guy

 

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From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of John Oreopoulos
Sent: Wednesday, 27 April 2011 1:14 AM
To: [hidden email]
Subject: Re: post aquization binning clarification

 

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Not exactly,

Richard pointed out that a true binning would sum the pixels only, and
not average them like the standard function does now. But I think the
original ImageJ plugin or function could be modified to do this simply
be removing the division part of the code. Richard, you could ask Wayne
Rasband if he could include this option in the dialog box in a future
version of ImageJ.

John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca


On 2011-04-26, at 9:57 AM, Arne Seitz wrote:

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>
> Hi Rich,
>
> your question has already been answered by John Oreopoulos yesterday.
>
> But in fact you do not need a pluging. If you use ImageJ 1.44 and

later you
> can use Image-->Scale...
> If you check "Average when downsizing" you get exactly what you were
looking
> for.
>
> Cheers Arne

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