Re: Live cell imaging with multiphoton confocal

Posted by Kate Luby-Phelps on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-with-multiphoton-confocal-tp6297623p6316779.html

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We have used 2P excitation successfully to selectively uncage in cells growing on
a feeder layer without uncaging in the feeder cells. Also, in single cells in a
cluster of pancreatic acinar cells. Inherent confocality of the focal volume in 2P
is an advantage in cases like that. I don't think this would have been possible
with 1P.