Posted by
Armstrong, Brian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-with-multiphoton-confocal-tp6297623p6317366.html
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Hi Kate, when you do these experiments are you using two lasers; uncaging with one wavelength, and imaging with another wavelength?
Brian D Armstrong PhD
Assistant Research Professor
Light Microscopy Core
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Kate Luby-Phelps
Sent: Friday, April 29, 2011 5:58 AM
To:
[hidden email]
Subject: Re: Live cell imaging with multiphoton confocal
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We have used 2P excitation successfully to selectively uncage in cells growing on
a feeder layer without uncaging in the feeder cells. Also, in single cells in a
cluster of pancreatic acinar cells. Inherent confocality of the focal volume in 2P
is an advantage in cases like that. I don't think this would have been possible
with 1P.
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