Re: Live cell imaging with multiphoton confocal

Posted by Kate Luby-Phelps on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-with-multiphoton-confocal-tp6297623p6321898.html

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Hi Mark,

Certainly for cultured cells and not too thick specimens, your way is a good
choice and I appreciate you bringing it to my attention. For thick or turbid
tissues or live animals, I think we will need 2P to image the probe after
photoactivation. We have a user who designs 2P photoactivatable probes and we
don't observe significant additional photolysis at the imaging wavelength.

Kate