Keeping IMF slides good for long period?

Posted by DamirSudar on
URL: http://confocal-microscopy-list.275.s1.nabble.com/CYTO2011-Pre-congress-courses-announcement-Fundamentals-of-Image-Cytometry-before-ISAC-2011-in-Baltie-tp6322453p6328239.html

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Dear all,

I would really appreciate some good suggestions how to keep
immunofluorescently labeled slides of mammalian cells good for long
periods (3-5 months). The fluors are typically Alexa dyes or similar. Is
freezing at -20 or -80 a good idea? Any preparation tricks that keep the
fluorescence and the localization intact?

Thanks,
- Damir

--
Damir Sudar - Staff Scientist and Deputy for Technology
Lawrence Berkeley Laboratory / Life Sciences Division
One Cyclotron Road, MS 977R225A, Berkeley, CA 94720, USA
T: 510/486-5346 - F: 510/486-5586 - E: [hidden email]
WWW: http://www.lbl.gov/lifesciences/labs/sudar_lab.html