Posted by
Greg Martin-8 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/CYTO2011-Pre-congress-courses-announcement-Fundamentals-of-Image-Cytometry-before-ISAC-2011-in-Baltie-tp6322453p6328278.html
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Hey Damir et.al. --
Fix your preps briefly with PFA after the final post-secondary rinse. Loss of signal in storage is usually due to the antibodies coming off. A quick fix will help to "lock" the antibodies in place.
Be peace! Greg.
Greg Martin
Keck Microscopy Facility
University of Washington School of Medicine
www.depts.washington.edu/keck
206-685-8784 (office)
425-344-2632 (cell)
----- Original Message -----
From: "Damir Sudar" <
[hidden email]>
To: <
[hidden email]>
Sent: Tuesday, May 03, 2011 10:58 AM
Subject: Keeping IMF slides good for long period?
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Dear all,
>
> I would really appreciate some good suggestions how to keep
> immunofluorescently labeled slides of mammalian cells good for long
> periods (3-5 months). The fluors are typically Alexa dyes or similar. Is
> freezing at -20 or -80 a good idea? Any preparation tricks that keep the
> fluorescence and the localization intact?
>
> Thanks,
> - Damir
>
> --
> Damir Sudar - Staff Scientist and Deputy for Technology
> Lawrence Berkeley Laboratory / Life Sciences Division
> One Cyclotron Road, MS 977R225A, Berkeley, CA 94720, USA
> T: 510/486-5346 - F: 510/486-5586 - E:
[hidden email]
> WWW:
http://www.lbl.gov/lifesciences/labs/sudar_lab.html>