Posted by
Armstrong, Brian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-with-multiphoton-confocal-tp6297623p6331395.html
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Hello Kate, would you be willing to share what 2P system, and what lasers, you chose?
What factors determined your decisions?
Thanks in advance,
We currently have a Prairie Ultima that we ordered with the second beam path. However, we have yet to add the second laser.
Brian D Armstrong PhD
Assistant Research Professor
Light Microscopy Core
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Kate Luby-Phelps
Sent: Saturday, April 30, 2011 6:06 AM
To:
[hidden email]
Subject: Re: Live cell imaging with multiphoton confocal
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So far we have been using one laser, which means we miss the beginning of the
timecourse after photoactivation because it takes several seconds for the Ti:S to
tune and mode-lock. So the experiments where 2P photoactivation has been
useful involve diffusion through gap junctions between cells in a cluster or chain
because the spread of the photoactivated dye between cells is relatively slow.
However, in June we will take delivery on a new 2P system with two Ti:S lasers
so we can do it simultaneously at two different wavelengths.
Kate
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