405 nm laser via NIR port on Zeiss 510

Posted by Phillips, Thomas E. on
URL: http://confocal-microscopy-list.275.s1.nabble.com/405-nm-laser-via-NIR-port-on-Zeiss-510-tp6345367.html

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The NIR laser for two photon excitation on our Zeiss 510 died on us. The repair would be at least 60K. We are trying to find funds for a new confocal so I don't know if it is worth repairing. The biggest practical problem is that we can't do DAPI excitation on our routine preps.  Has anyone tried retrofitting a 405 nm laser through the NIR port?  Are there optical components/mirrors in the pathway that only work with longer wavelengths? Any thoughts on this are welcome. Tom


Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
[hidden email]<mailto:[hidden email]>

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/