Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/405-nm-laser-via-NIR-port-on-Zeiss-510-tp6345367p6345782.html
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For starters, what do you have for a Dichroic that could pipe in the 405 and
let the rest of the visible pass? Most 2p dichroics split off at around 680
or 700nm to allow the IR to pass and the emission light to reflect, or
vice-versa.
Craig
On Mon, May 9, 2011 at 1:16 PM, Phillips, Thomas E.
<
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> The NIR laser for two photon excitation on our Zeiss 510 died on us. The
> repair would be at least 60K. We are trying to find funds for a new confocal
> so I don't know if it is worth repairing. The biggest practical problem is
> that we can't do DAPI excitation on our routine preps. Has anyone tried
> retrofitting a 405 nm laser through the NIR port? Are there optical
> components/mirrors in the pathway that only work with longer wavelengths?
> Any thoughts on this are welcome. Tom
>
>
> Thomas E. Phillips, Ph.D
> Professor of Biological Sciences
> Director, Molecular Cytology Core
> 2 Tucker Hall
> University of Missouri
> Columbia, MO 65211-7400
> 573-882-4712 (office)
> 573-882-0123 (fax)
>
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