Posted by
Armstrong, Brian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/405-nm-laser-via-NIR-port-on-Zeiss-510-tp6345367p6345841.html
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Craig makes a good point. The HFT700/488 or KP650 (for example) that you have been using for your 2P laser will not work for a 405 line. Therefore you would need to change the dichroics. This is not done in the field and you would need to send your scan-head to the factory in Germany. You would also need to think about how to get your laser into the fiber (AOTF?), how to adjust the power (AOM is no longer useful), and whether or not you have the secondary dichroics that will pass the emitted light.
You should talk to Zeiss before considering this idea any further.
Cheers,
Brian D Armstrong PhD
Assistant Research Professor
Light Microscopy Core
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Craig Brideau
Sent: Monday, May 09, 2011 2:52 PM
To:
[hidden email]
Subject: Re: 405 nm laser via NIR port on Zeiss 510
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For starters, what do you have for a Dichroic that could pipe in the 405 and
let the rest of the visible pass? Most 2p dichroics split off at around 680
or 700nm to allow the IR to pass and the emission light to reflect, or
vice-versa.
Craig
On Mon, May 9, 2011 at 1:16 PM, Phillips, Thomas E.
<
[hidden email]>wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> The NIR laser for two photon excitation on our Zeiss 510 died on us. The
> repair would be at least 60K. We are trying to find funds for a new confocal
> so I don't know if it is worth repairing. The biggest practical problem is
> that we can't do DAPI excitation on our routine preps. Has anyone tried
> retrofitting a 405 nm laser through the NIR port? Are there optical
> components/mirrors in the pathway that only work with longer wavelengths?
> Any thoughts on this are welcome. Tom
>
>
> Thomas E. Phillips, Ph.D
> Professor of Biological Sciences
> Director, Molecular Cytology Core
> 2 Tucker Hall
> University of Missouri
> Columbia, MO 65211-7400
> 573-882-4712 (office)
> 573-882-0123 (fax)
>
[hidden email]<mailto:
[hidden email]>
>
>
http://www.biology.missouri.edu/faculty/phillips.html>
http://www.biotech.missouri.edu/mcc/>
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