Re: 405 nm laser via NIR port on Zeiss 510

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/405-nm-laser-via-NIR-port-on-Zeiss-510-tp6345367p6346027.html

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The primary dichroic is typically the largest problem.  Our Nikon technician
went through some fairly heroic efforts to install a dichroic into an A1
FN1.  We had the filter custom made at significant expense for our
ultrabroadband Ti:Saph laser (Octavius), so the possibility of damaging the
filter during installation was a critical concern.  After the technician
partly disassembled the scan head in our lab, we ended up drafting a nice
lady with very tiny fingers who did needlepoint and crocheting as a hobby to
finally get the filter into place in the A1 scan head.  This was something
normally done at the factory...  That said, it did work out for us and Nikon
was very accommodating in helping us with the modification.
Regarding piping the laser in to the Zeiss scope: I was assuming your laser
could be brought in free space.  If you have to go through a fiber then you
probably won't be able to do it due to dispersion in the glass fiber.  If
you have a prechirping unit then you *might* be able to pull it off, but it
will be tricky.

Craig



On Mon, May 9, 2011 at 4:06 PM, Armstrong, Brian <[hidden email]> wrote:

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>
> Craig makes a good point. The HFT700/488 or KP650 (for example) that you
> have been using for your 2P laser will not work for a 405 line. Therefore
> you would need to change the dichroics. This is not done in the field and
> you would need to send your scan-head to the factory in Germany. You would
> also need to think about how to get your laser into the fiber (AOTF?), how
> to adjust the power (AOM is no longer useful), and whether or not you have
> the secondary dichroics that will pass the emitted light.
>
> You should talk to Zeiss before considering this idea any further.
>
> Cheers,
>
>
> Brian D Armstrong PhD
> Assistant Research Professor
> Light Microscopy Core
> Beckman Research Institute
> City of Hope
> Dept of Neuroscience
> 1450 E Duarte Rd
> Duarte, CA 91010
> 626-256-4673 x62872
>
>
> http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Craig Brideau
> Sent: Monday, May 09, 2011 2:52 PM
> To: [hidden email]
> Subject: Re: 405 nm laser via NIR port on Zeiss 510
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> For starters, what do you have for a Dichroic that could pipe in the 405
> and
> let the rest of the visible pass?  Most 2p dichroics split off at around
> 680
> or 700nm to allow the IR to pass and the emission light to reflect, or
> vice-versa.
>
> Craig
>
>
> On Mon, May 9, 2011 at 1:16 PM, Phillips, Thomas E.
> <[hidden email]>wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > The NIR laser for two photon excitation on our Zeiss 510 died on us. The
> > repair would be at least 60K. We are trying to find funds for a new
> confocal
> > so I don't know if it is worth repairing. The biggest practical problem
> is
> > that we can't do DAPI excitation on our routine preps.  Has anyone tried
> > retrofitting a 405 nm laser through the NIR port?  Are there optical
> > components/mirrors in the pathway that only work with longer wavelengths?
> > Any thoughts on this are welcome. Tom
> >
> >
> > Thomas E. Phillips, Ph.D
> > Professor of Biological Sciences
> > Director, Molecular Cytology Core
> > 2 Tucker Hall
> > University of Missouri
> > Columbia, MO 65211-7400
> > 573-882-4712 (office)
> > 573-882-0123 (fax)
> > [hidden email]<mailto:[hidden email]>
> >
> > http://www.biology.missouri.edu/faculty/phillips.html
> > http://www.biotech.missouri.edu/mcc/
> >
>
>
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