Posted by
Sonja Hatz on
URL: http://confocal-microscopy-list.275.s1.nabble.com/bubbling-media-and-imaging-tp593362p639940.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHi Simon,
If you are just after a hypoxic media, it should be easiest and best
to work under an atmosphere of nitrogen, rather than bubbling your
samples - the concentration in your solution will depend on the
solubility of oxygen in the media and the oxygen concentration in the
atmosphere (Henry's law). As far as I know you will not be able to
remove 100% oxygen from aqueous solutions anyway (without freezing
and/or high vacuum), so you may as well work with nearly oxygen free
media. We use a mini-incubation chamber, sold by lots of suppliers,
but a petidish with holes for gas inlet and outlet could also be
sufficient.
Sonja
Citat af "Watkins, Simon C" <
[hidden email]>:
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> Hi folks, I in the middle of a bunch of experiments where I need to
> bubble gas into media while imaging (Going from normoxic to hypoxic
> media). Obviously the bubbles perturb the quality of the DIC image.
> I have tried all sorts of homemade diffusers to minimize the
> effect but to no avail... So has anyone of you developed a solution
> to this problem? I could exchange the media, however regassing
> happens really quickly and the effects we are measuring are subtle.
> Thus any changes in ionic concentrations or temperature may lead
> to a similar effect.
> Ideas anyone
> simon
>
> Simon C. Watkins Ph.D, FRCPath
> Professor and Vice Chair, Cell Biology and Physiology
> Professor, Immunology
> Director, Center for Biologic Imaging
> BSTS 225, University of Pittsburgh
> 3500 Terrace St.
> Pittsburgh PA 15261
> Tel: 412-352-2277
> Fax:412-648-2797
> URL:
http://www.cbi.pitt.edu>
>
Sonja Hatz
Department of Chemistry
University of Aarhus
Langelandsgade 140
DK-8000 Aarhus C
Denmark
Tel: (45) 8942 3860
Fax: (45) 8619 6199
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