Fluorophore excitable with 405nm and 488nm lines

Posted by Daniel Gitler on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluorophore-excitable-with-405nm-and-488nm-lines-tp6432486.html

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Dear Confocalists/microscopists,
I am in need of either beads or a fluorophore in solution which is exited consistently by both the 405nm and 488nm lines of a confocal microscope. What I really need is that the ratio of excitation should be constant, in which case two separate dyes are probably not a good choice, unless their molar ratio can be quite consistent (perhaps in beads?). I also need that the efficiency of excitation for both lines be quite decent (doesn't have to be maximal, just decent). Finally (it appears I have a lot of requisites) the dye/beads should be as insensitive as possible to environmental changes (i.e., pH etc). The idea is to have a good standard in order to calibrate the ratio of the power of these two lines when imaging a sample (I do not need to know the actual number, just a relative measure will do fine).
I would appreciate any advice,
Thanks,
Daniel


Daniel Gitler, Ph.D.
Department of Physiology and Neurobiology
Faculty of Health Sciences
Ben Gurion University of the Negev
Beer-Sheva 84105
Israel

Tel:  [phone]
Cell: [phone]
Fax: + 972-8-6477628
http://web2.bgu.ac.il/physiology/faculty-members/daniel-gitler/‎