Re: Fluorophore excitable with 405nm and 488nm lines

Posted by Yuval Ebenstein on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluorophore-excitable-with-405nm-and-488nm-lines-tp6432486p6433999.html

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We routinely excite red emitting quantum dots with both 405 and 488.
choose the emission that suits you best and you can excite anywhere to
the blue of the emission. The 625nm are the brightest from my experience.
You can also embed them in polymer to make an everlasting calibration
sample and you can tune the concentration down to the single quantum dot
level for PSF measurements.
Hope this helps
Yuval

On 6/2/2011 11:59 AM, Daniel Gitler wrote:

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> Dear Confocalists/microscopists,
> I am in need of either beads or a fluorophore in solution which is exited consistently by both the 405nm and 488nm lines of a confocal microscope. What I really need is that the ratio of excitation should be constant, in which case two separate dyes are probably not a good choice, unless their molar ratio can be quite consistent (perhaps in beads?). I also need that the efficiency of excitation for both lines be quite decent (doesn't have to be maximal, just decent). Finally (it appears I have a lot of requisites) the dye/beads should be as insensitive as possible to environmental changes (i.e., pH etc). The idea is to have a good standard in order to calibrate the ratio of the power of these two lines when imaging a sample (I do not need to know the actual number, just a relative measure will do fine).
> I would appreciate any advice,
> Thanks,
> Daniel
>
>
> Daniel Gitler, Ph.D.
> Department of Physiology and Neurobiology
> Faculty of Health Sciences
> Ben Gurion University of the Negev
> Beer-Sheva 84105
> Israel
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> Tel:  [phone]
> Cell: [phone]
> Fax: + 972-8-6477628
> http://web2.bgu.ac.il/physiology/faculty-members/daniel-gitler/‎

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Yuval Ebenstein
Department of Chemistry and Biochemistry, UCLA
Young Hall-2002, 607 Charles E. Young Drive East
Los Angeles, CA 90095-1569
Ph: [phone]; Fax: [phone]