Re: Fluorophore excitable with 405nm and 488nm lines

Posted by mcammer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluorophore-excitable-with-405nm-and-488nm-lines-tp6432486p6434832.html

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Depending on your tolerance, they may or may not be stable.  I use these all the time for aligning the microscope because they are great for this, although there may be an issue with refractive index mismatch especially with the newer TIRF objectives, but I'm not so sure as a reproducible sample.
Please see http://www.einstein.yu.edu/aif/instructions/fluor-ref-slides/01.htm and
http://www.einstein.yu.edu/aif/instructions/zeiss/liveduo/Zaxis_bleachingtest/index.htm
for two examples of bleaching.  
-Michael

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Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

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From: Confocal Microscopy List [[hidden email]] On Behalf Of Guy Cox [[hidden email]]
Sent: Thursday, June 02, 2011 11:32 PM
To: [hidden email]
Subject: Re: Fluorophore excitable with 405nm and 488nm lines

I just checked out a Chroma green fluorescent plastic slide - it seemed to fluoresce pretty well with both uv and blue LEDs so surely that should work well enough.  Doesn't have the ultra-long lifetime problem of uranyl glass.  Reproducible, robust and best of all free!

                                   Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
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Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

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