Re: Phalloidin background on transwell membrane

Posted by Ryan Schreiner on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Phalloidin-background-on-transwell-membrane-tp6474271p6474366.html

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We use polycarbonate transwells for fluorescent studies and do not
have problems with filter background, whereas the many of the clear
filters can have increased background signal, the CF series of
fluorophores coupled to phalloidin from Biotium are particularly good.

On Tue, Jun 14, 2011 at 10:30 AM, Renato Mortara <[hidden email]> wrote:

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> Dear all
>
> A colleague of mine is trying to label polarized epithelial cells grown on
> transwell dishes.
>
> However, fluorescent phalloidin is labeling the dish membrane, rendering it
> impossible to image the cells.
>
> She tried to block with goat serum, BSA with no luck.
>
> Any suggestions ?
>
> Many thanks !
>
> Renato
>
> Renato A. Mortara
> Parasitology Division
> UNIFESP - Escola Paulista de Medicina
> Rua Botucatu, 862, 6th floor
> São Paulo, SP
> 04023-062
> Brazil
> Phone: [phone]
> Fax:     [phone]
> email: [hidden email]
> home page: www.ecb.epm.br/~ramortara
>