Re: Phalloidin background on transwell membrane

Posted by Cameron, Lisa on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Phalloidin-background-on-transwell-membrane-tp6474271p6474970.html

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I would recommend looking at the dish membrane without any fluorophore labeling
to check for autofluorescence. I've had a couple people use membranes that are
autofluorescent - mostly in the 488nm range.
So red or far red phalloidin would be better in this case, perhaps.
- Lisa


---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy Core
Dana Farber Cancer Institute
Boston, MA 02215
[hidden email]


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Renato Mortara
Sent: Tuesday, June 14, 2011 10:30 AM
To: [hidden email]
Subject: [CONFOCALMICROSCOPY] Phalloidin background on transwell membrane

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Dear all
 
A colleague of mine is trying to label polarized epithelial cells grown on
transwell dishes.
 
However, fluorescent phalloidin is labeling the dish membrane, rendering it
impossible to image the cells.
 
She tried to block with goat serum, BSA with no luck.
 
Any suggestions ?
 
Many thanks !
 
Renato
 
Renato A. Mortara
Parasitology Division
UNIFESP - Escola Paulista de Medicina
Rua Botucatu, 862, 6th floor
São Paulo, SP
04023-062
Brazil
Phone: [phone]
Fax:     [phone]
email: [hidden email]
home page: www.ecb.epm.br/~ramortara


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