Re: Phalloidin background on transwell membrane

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Phalloidin-background-on-transwell-membrane-tp6474271p6481379.html

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use fluorescent anti-actin


On 6/14/2011 10:30 AM, Renato Mortara wrote:

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>
> Dear all
>
> A colleague of mine is trying to label polarized epithelial cells grown on
> transwell dishes.
>
> However, fluorescent phalloidin is labeling the dish membrane, rendering it
> impossible to image the cells.
>
> She tried to block with goat serum, BSA with no luck.
>
> Any suggestions ?
>
> Many thanks !
>
> Renato
>
> Renato A. Mortara
> Parasitology Division
> UNIFESP - Escola Paulista de Medicina
> Rua Botucatu, 862, 6th floor
> São Paulo, SP
> 04023-062
> Brazil
> Phone: [phone]
> Fax:     [phone]
> email: [hidden email]
> home page: www.ecb.epm.br/~ramortara
>
>    

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George McNamara, PhD
Analytical Imaging Core Facility
University of Miami