Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluorophore-excitable-with-405nm-and-488nm-lines-tp6432486p6481413.html
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Invitrogen/Molecular Probes TetraSpeck beads.
With respect to Uranyl glass -- you can get Uranium crystals from any
TEM lab or cell biology lab that does a lot of TEM. And yes, sticking a
Geiger counter over an open bottle results in more clicks than elsewhere
in a lab. My thanks to Gregg Gundersen for demonstrating this.
George
On 6/2/2011 2:59 PM, Daniel Gitler wrote:
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> Dear Confocalists/microscopists,
> I am in need of either beads or a fluorophore in solution which is exited consistently by both the 405nm and 488nm lines of a confocal microscope. What I really need is that the ratio of excitation should be constant, in which case two separate dyes are probably not a good choice, unless their molar ratio can be quite consistent (perhaps in beads?). I also need that the efficiency of excitation for both lines be quite decent (doesn't have to be maximal, just decent). Finally (it appears I have a lot of requisites) the dye/beads should be as insensitive as possible to environmental changes (i.e., pH etc). The idea is to have a good standard in order to calibrate the ratio of the power of these two lines when imaging a sample (I do not need to know the actual number, just a relative measure will do fine).
> I would appreciate any advice,
> Thanks,
> Daniel
>
>
> Daniel Gitler, Ph.D.
> Department of Physiology and Neurobiology
> Faculty of Health Sciences
> Ben Gurion University of the Negev
> Beer-Sheva 84105
> Israel
>
> Tel: +972-8-6477345
> Cell: +972-54-2110100
> Fax: + 972-8-6477628
>
http://web2.bgu.ac.il/physiology/faculty-members/daniel-gitler/>
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--
George McNamara, PhD
Analytical Imaging Core Facility
University of Miami