Re: Deconvolving Spinning Disk Images
Posted by Dani Bodor on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolving-Spinning-Disk-Images-tp6508446p6510145.html
> David: that is a point I had also already considered. Maybe it is in fact best to use the measured "ghosted" PSF for deconvolution. As I had already mentioned initially, the ghosts are much much less bright than the focal point, so in my images it will probably not be a huge problem. I am just afraid that small errors in the PSF measurements might heavily screw up the deconvolution process.
> Getta: thank you very much for your suggestions, i will try to find some samples pictures to send you so that you could take a look and hopefully advise me further.