Re: Live fixable fluorescent cell labelling

Posted by JOEL B. SHEFFIELD on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolving-Spinning-Disk-Images-tp6508446p6512717.html

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I just looked over the paper, and I see that we were  able to retain label
only for two days or so because, we assumed, of protein turnover in the
cells.  This may be a fundamental problem that you will have to consider.
You may want to consider a fluorescent protein label that could be
transfected in.

Joel


On Fri, Jun 24, 2011 at 12:44 PM, Michal Opas <[hidden email]> wrote:

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> Right!
> Thanks Joel,
>
> Michal
>

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Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs