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Re: Live fixable fluorescent cell labelling

Posted by Kilgore, Jason-2 on Jun 24, 2011; 5:10pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Deconvolving-Spinning-Disk-Images-tp6508446p6512772.html

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Likely TRITC and FITC would be surface labels, which might have higher protein turnover.  Another drawback of that method, for some assays, would be the potential disruption of cell surface protein function after the conjugation, potentially affecting cell-cell interactions.


Jason

Jason A. Kilgore
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Molecular Probes Labeling and Detection Technologies
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of JOEL B. SHEFFIELD
Sent: Friday, June 24, 2011 9:50 AM
To: [hidden email]
Subject: Re: Live fixable fluorescent cell labelling

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I just looked over the paper, and I see that we were  able to retain label
only for two days or so because, we assumed, of protein turnover in the
cells.  This may be a fundamental problem that you will have to consider.
You may want to consider a fluorescent protein label that could be
transfected in.

Joel


On Fri, Jun 24, 2011 at 12:44 PM, Michal Opas <[hidden email]> wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
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>
> Right!
> Thanks Joel,
>
> Michal
>



--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs