Concentrating bacteria cells for microscope visualization.

Posted by Tom Lawson on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Concentrating-bacteria-cells-for-microscope-visualization-tp6519025.html

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Dear Confocalists,

I am having a few problems separating and concentrating bacteria cells for
microscope visualization.

I have a 1 ml solution in an aliquot that contains some eukaryote debris and
10 to 100 bacteria cells. After spotting the centrifuge concentrate to a
slide-well, I can see the bacteria with a nucleic stain. But the debris in
the concentrate makes it hard to pipette and blocks seeing the bacteria. In
addition, the concentrate is spread over a 6 mm slide-well which makes it
hard to locate the bacteria.

I would be grateful for any suggestions.

Regards,

--
Tom Lawson
[hidden email]
PhD Student,
Macquarie University
NSW, 2109
Australia