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Kilgore, Jason-2 on
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Tom, though it won't help with microscopic examination, have you considered using a microplate reader format? You could use a nucleic acid stain, such as Hoechst 33342, and compare the signal to a standard curve generated from wells with known bacterial densities.
Jason
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Jason A. Kilgore
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Molecular Probes Labeling and Detection Technologies
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Tom Lawson
Sent: Sunday, June 26, 2011 9:45 PM
To:
[hidden email]
Subject: Concentrating bacteria cells for microscope visualization.
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Dear Confocalists,
I am having a few problems separating and concentrating bacteria cells for
microscope visualization.
I have a 1 ml solution in an aliquot that contains some eukaryote debris and
10 to 100 bacteria cells. After spotting the centrifuge concentrate to a
slide-well, I can see the bacteria with a nucleic stain. But the debris in
the concentrate makes it hard to pipette and blocks seeing the bacteria. In
addition, the concentrate is spread over a 6 mm slide-well which makes it
hard to locate the bacteria.
I would be grateful for any suggestions.
Regards,
--
Tom Lawson
[hidden email]
PhD Student,
Macquarie University
NSW, 2109
Australia