Posted by
"Brüne Venus" on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Concentrating-bacteria-cells-for-microscope-visualization-tp6519025p6526489.html
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Hi Tom,
Since a couple of years now Jakob Pernthaler concentrates bacteria using a filter approach and afterwards detects them using fluorescent markers like DAPI and even does FISH. For data acquisition he uses an upright fluorescence microscope with scanning stage. He is now based in Zuerich Switzerland www.limnology.ch - or pubmed.
Best regards
Bruene Venus
Company affiliation:
Carl Zeiss MicroImaging Germany
Mailto:
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-------- Original-Nachricht --------
> Datum: Mon, 27 Jun 2011 14:44:51 +1000
> Von: Tom Lawson <
[hidden email]>
> An:
[hidden email]
> Betreff: Concentrating bacteria cells for microscope visualization.
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Dear Confocalists,
>
> I am having a few problems separating and concentrating bacteria cells for
> microscope visualization.
>
> I have a 1 ml solution in an aliquot that contains some eukaryote debris
> and
> 10 to 100 bacteria cells. After spotting the centrifuge concentrate to a
> slide-well, I can see the bacteria with a nucleic stain. But the debris in
> the concentrate makes it hard to pipette and blocks seeing the bacteria.
> In
> addition, the concentrate is spread over a 6 mm slide-well which makes it
> hard to locate the bacteria.
>
> I would be grateful for any suggestions.
>
> Regards,
>
> --
> Tom Lawson
>
[hidden email]
> PhD Student,
> Macquarie University
> NSW, 2109
> Australia
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