Posted by
Kurt Thorn on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Third-FP-options-CFP-alternatives-tp6549503p6550740.html
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We've have good luck with using TagBFP excited by 405nm light in
conjunction with GFP and mCherry. An improved TagBFP2 is supposed to be
coming soon. In the far-red channel, some of our users have had good
luck with the recently published IFP1.4, although this requires
biliverdin as a cofactor. It's readily excited at 640nm.
Best,
Kurt
On 7/5/2011 5:18 AM, Adrian Smith wrote:
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>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hi all,
>
> One of our users is looking to set up with a third fluorescent protein for some imaging studies (confocal and multiphoton).
>
> He currently has components labelled with GFP (or YFP in some experiments) and mCherry so he is considering something either in the BFP/CFP range or potentially in the red range past mCherry.
>
> I'm interested in people thoughts on what are the best options these days? There seems to be bewildering array of newer FPs available...
>
> (eg the Evrogen Turbo/Tag reagents like TurboFP650/TagBFP/TagCFP look good but may not work as needs to be able to subclone in order to get into a Leishmania parasite).
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>
>