http://confocal-microscopy-list.275.s1.nabble.com/Third-FP-options-CFP-alternatives-tp6549503p6551505.html
I have also worked with TagBFP to good effect in neurons with the 405nm laser. It is very well separated from EGFP, and did not show any cross excitation with the 488nm laser.
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>
> We've have good luck with using TagBFP excited by 405nm
> light in
> conjunction with GFP and mCherry. An improved TagBFP2 is
> supposed to be
> coming soon. In the far-red channel, some of our users
> have had good
> luck with the recently published IFP1.4, although this requires
> biliverdin as a cofactor. It's readily excited at 640nm.
>
> Best,
> Kurt
>
> On 7/5/2011 5:18 AM, Adrian Smith wrote:
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> >
> > Hi all,
> >
> > One of our users is looking to set up with a third fluorescent
> protein for some imaging studies (confocal and multiphoton).
> >
> > He currently has components labelled with GFP (or YFP in some
> experiments) and mCherry so he is considering something either
> in the BFP/CFP range or potentially in the red range past mCherry.
> >
> > I'm interested in people thoughts on what are the best options
> these days? There seems to be bewildering array of newer FPs
> available...>
> > (eg the Evrogen Turbo/Tag reagents like
> TurboFP650/TagBFP/TagCFP look good but may not work as needs to
> be able to subclone in order to get into a Leishmania parasite).
> >
> > Regards,
> >
> > Adrian Smith
> > Centenary Institute, Sydney, Australia
> >
> >
>